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A transposable element annotation pipeline and expression analysis reveal potentially active elements in the microalga Tisochrysis lutea ArchiMer
Berthelier, Jeremy; Casse, Nathalie; Daccord, Nicolas; Jamilloux, Veronique; Saint-jean, Bruno; Carrier, Gregory.
Background Transposable elements (TEs) are mobile DNA sequences known as drivers of genome evolution. Their impacts have been widely studied in animals, plants and insects, but little is known about them in microalgae. In a previous study, we compared the genetic polymorphisms between strains of the haptophyte microalga Tisochrysis lutea and suggested the involvement of active autonomous TEs in their genome evolution. Results To identify potentially autonomous TEs, we designed a pipeline named PiRATE (Pipeline to Retrieve and Annotate Transposable Elements, download: https://doi.org/10.17882/51795), and conducted an accurate TE annotation on a new genome assembly of T. lutea. PiRATE is composed of detection, classification and annotation steps. Its...
Tipo: Text Palavras-chave: Transposable elements; Genome assembly; Pipeline; Tool; Annotation; Algae; Haptophyte; Tisochrysis lutea.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00441/55250/56732.pdf
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Vertical and horizontal controls of a haptophyte thin layer in the Bay of Biscay, France ArchiMer
Farrell, Hazel; Gentien, Patrick; Fernand, Liam; Lazure, Pascal; Lunven, Michel; Youenou, Agnes; Reguera, Beatriz; Raine, Robin.
Investigations into the phytoplankton of the Bay of Biscay in July 2006 revealed an extensive bloom of an unidentified haptophyte species. High-resolution sampling techniques identified the region in the vicinity of the mouth of the Loire estuary as an area of high (106–107 cells l−1) cell densities of the haptophyte in a thin sub-surface layer. This organism dominated the phytoplankton population regionally. Measurements of the vertical distribution of horizontal shear showed that the population, located at ca. 20 m depth, was confined to a layer lying between two density discontinuities with high shear. Physical measurements indicated that the population was being advected southeastwards, along the coast. Seaward spreading of the population was also...
Tipo: Text Palavras-chave: Haptophyte; Bay of Biscay; Horizontal dispersal; Thin layers.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00160/27129/25327.pdf
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